CULTIVATION PARAMETERS OF THE “BIEMG-51” VACCINIA VIRUS STRAIN IN DEVELOPING CHICKEN EMBRYOS
DOI:
https://doi.org/10.52754/16948610_2026_3_5%20Keywords:
vaccinia virus, virus cultivation, developing chicken embryos, chorioallantoic membrane, dose, biological activityAbstract
The article presents the results of studies aimed at determining the optimal cultivation parameters of the “BIEMG-51” vaccinia virus strain in developing chicken embryos on the chorioallantoic membrane. The relevance of the study is associated with the need to obtain highly active virus-containing material for the development of diagnostic and preventive tools against orthopoxvirus infections. The aim of the study was to determine the optimal conditions for cultivating the “BIEMG-51” vaccinia virus strain in developing chicken embryos. In the study, 9–13-day-old chicken embryos were infected with virus doses ranging from 10 to 100000 EID50/0.2 cm³. Incubation was carried out at temperatures of (33 ± 1) °C, (35 ± 1) °C, and (37 ± 1) °C for 24–144 hours. The biological activity of the virus was determined by titration with calculation of EID50 according to the Reed & Muench method. The optimal cultivation conditions were found to be an infectious dose of 100 EID50/0.2 cm³, the use of 11–12-day-old embryos, an incubation temperature of (37 ± 1) °C, and a cultivation period of 120 hours. Under these conditions, the infectious activity of the virus reached 6.50±0.08 lg EID50/cm³.
References
Абдураимов, Е.О., Мамбеталиев, М., & Кутумбетов, Л.Б. (1998). Изучение свойств вируса оспы коз в клеточных культурах. Биотехнология: теория и практика, 1–2(5–6), 9–11.
Бобина, Е.А., Корниенко, Е.И., & Горковенко, Н.Е. (2018). Биологические системы для культивирования вирусов. Стратегии и тренды развития науки в современных условиях, 1(4), 4–7.
Борисевич, Ю.Ф., & Орехов, М.Д. (1966). Оспа верблюдов. Ветеринария, 3, 50–53.
Волонтырь, А.В., Сарыгина, Е.В., Просвирова, К.А., & Потапова, А.Ю. (2019). Куриный эмбрион как модельный объект в биологии. Волгоград, Россия, 1–4.
Вора, Н.М., Гелеишвили, М., Хмаладзе, Э., Маглакелидзе, Г., Навдарашвили, А. (2016). Заражение человека зоонозным ортопоксвирусом в Грузии. New England Journal of Medicine, 375, 1223–1230.
Диев, В.И., Захаров, В.М., & Рахманов, А.М. (2003). Оспа овец и коз: Эпизоотическая ситуация и профилактика. Ветеринария, 11, 3-6.
Кутумбетов, Л.Б., & Мырзахметова, Б.Ш. (2021). Оспенные болезни животных и биотехнология изготовления средств специфической профилактики. Алматы.
Мейхи, Б. (Ред.). (1988). Вирусология. Методы. Москва: Мир.
Сюрин, В.Н., Самуйленко, А.Я., Соловьев, Б.В., & Фомина, Н.В. (1998). Вирусные болезни животных. Москва: ВНИТИБП.
Bessarabov, B.F. (2006). Incubation of eggs with the basics of poultry embryology. Moscow: KolosS.
Chantrey, J., Meyer, H., Baxby, D., Begon, M., Bown, K. J., Hazel, S. M., et al. (1999). Cowpox: Reservoir hosts and geographic range. Epidemiology and Infection, 122(3), 455–460.
de Oliveira, J.S., Figueiredo, P.O., Costa, G.B., De Assis, F.L., Drumond, B.P., Da Fonseca, F.G., et al. (2017). Vaccinia virus natural infections in Brazil: The good, the bad, and the ugly. Viruses, 9(11), 340.
Dieterlen-Lièvre, F., Le Douarin, N. M. (2004). From the hemangioblast to self-tolerance: A series of innovations gained from studies on the avian embryo. Mechanisms of Development, 121(9), 1117–1128.
Doshi, R.H., Guagliardo, S.A J., Doty, J.B., Babeaux, A.D., Matheny, A., Burgado, J., et al. (2019). Epidemiologic and ecologic investigations of monkeypox, Likouala Department, Republic of the Congo, 2017. Emerging Infectious Diseases, 25(2), 253–273.
Dubois, M.E., & Slifka, M.K. (2008). Retrospective analysis of monkeypox infection. Emerging Infectious Diseases, 14(4), 592–599. https://doi.org/10.3201/eid1404.071044
Ducournau, C., Ferrier-Rembert, A., Ferraris, O., Joffre, A., Favier, A. L., Flusin, O., et al. (2013). Concomitant human infections with 2 cowpox virus strains in related cases, France, 2011. Emerging Infectious Diseases, 19(12), 1996–1998. https://doi.org/10.3201/eid1912.130256
Franco-Luiz, A.P. M., Fagundes-Pereira, A., Costa, G.B., Alves, P. A., Oliveira, D.B., Bonjardim, C.A., et al. (2014). Spread of vaccinia virus to cattle herds, Argentina, 2011. Emerging Infectious Diseases, 20(9), 1576–1578. https://doi.org/10.3201/eid2009.140353
Franco-Luiz, A.P.M., Oliveira, D.B., Pereira, A.F., Gasparini, M.C.S., Bonjardim, C.A., Ferreira, P.C.P., et al. (2016). Detection of vaccinia virus in dairy cattle serum samples from 2009, Uruguay. Emerging Infectious Diseases, 22(12), 2174–2176. https://doi.org/10.3201/eid2212.151916
Haller, S.L., Peng, C., McFadden, G., & Rothenburg, S. (2014). Poxviruses and the evolution of host range and virulence. Infection, Genetics and Evolution, 21, 15–40. https://doi.org/10.1016/j.meegid.2013.10.008
Holmes, D.J., & Ottinger, M.A. (2003). Birds as long-lived animal models for the study of aging. Experimental Gerontology, 38(11–12), 1365–1375.
Tretyakov, N.P., Bessarabov, B.F., & Krok, G.S. (1990). Inkubatsiya s osnovami embriologii [Incubation with the Basics of Embryology]. Moscow: Agropromizdat.
Ninove, L., Domart, Y., Vervel, C., Voinot, C., Salez, N., Raoult, D., et al. (2009). Cowpox virus transmission from pet rats to humans, France. Emerging Infectious Diseases, 15(5), 781–784. https://doi.org/10.3201/eid1505.090235
Rimoin, A.W., Mulembakani, P.M., Johnston, S.C., Lloyd-Smith, J.O., Kisalu, N.K., Kinkela, T.L., et al. (2010). Major increase in human monkeypox incidence 30 years after smallpox vaccination campaigns cease in the Democratic Republic of Congo. Proceedings of the National Academy of Sciences of the United States of America, 107(37), 16262–16267. https://doi.org/10.1073/pnas.1005769107
Singh, R.K., Balamurugan, V., Bhanuprakash, V., Venkatesan, G., & Hosamani, M. (2012). Emergence and reemergence of vaccinia-like viruses: Global scenario and perspectives. Indian Journal of Virology, 28, 1–11.
Stern, C.D. (2006). Neural induction: 10 years on since the “default model”. Current Opinion in Cell Biology, 18(6), 692–697.
Stern, C.D. (2018). The chick model system: A distinguished past and a great future. International Journal of Developmental Biology, 62(1–2–3–4), 1–4.
Usme-Ciro, J.A., Paredes, A., Walteros, D.M., Tolosa-Pérez, E.N., Laiton-Donato, K., Pinzón, M.C., et al. (2017). Detection and molecular characterization of zoonotic poxviruses circulating in the Amazon region of Colombia, 2014. Emerging Infectious Diseases, 23(4), 649–652. https://doi.org/10.3201/eid2304.161565
Vasiliev, Y.M. (2010). Optimization of reproduction of avian influenza viruses in various substrates and improvement of inactivated vaccines against avian influenza virus (Candidate dissertation). Research Institute of Vaccines and Serums named after I. I. Mechnikov RAS, Moscow.
Venkatesan, G., Balamurugan, V., Prabhu, M., Yogisharadhya, R., Bora, D.P., Gandhale, P. N., et al. (2010). Emerging and re-emerging zoonotic buffalopox infection: A severe outbreak in Kolhapur (Maharashtra), India. Veterinaria Italiana, 46, 439–448.
Vogel, S., Sárdy, M., Glos, K., Korting, H. C., Ruzicka, T., & Wollenberg, A. (2012). The Munich outbreak of cutaneous cowpox infection: Transmission by infected pet rats. Acta Dermato-Venereologica, 92, 126–131.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2026 Алина Алиева, Асанкадыр Жунушов , Аида Бердибаева, Санат Килибаев , Молдир Азанбекова , Кайнар Баракбаев , Куандык Жугунисов
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
