Parameters of oxidative stress and endogenous intoxication in experimental modeling of destructive cholecystitis
DOI:
https://doi.org/10.52754/16948610_2026_1_5Keywords:
oxidative stress, endogenous intoxication, destructive cholecystitis, lipid peroxidation, antioxidant systemAbstract
Relevance. This article discusses how current data highlight the importance of systemic disturbances, including oxidative stress (OS) and endogenous intoxication (EI), in the pathogenesis of ADC. The purpose of this article is to study the temporal dynamics of OS and EI parameters in an experimental model in 60 rabbits divided into 5 groups (days 0, 2, 5, 7, and 10). ADC was simulated by cystic duct ligation and the introduction of a bacterial suspension. Lipid peroxidation markers (DK, CT, MDA), antioxidant system activity (AOA, catalase), EI indices (SMP, phospholipase A2), and phospholipid composition were determined. By day 7, an increase in lipid peroxidation (DK, CT, MDA ↑ 2.9–5.4 times), depletion of AOS (AOA and catalase ↓ 3 times), an increase in SMP (2 times) and phospholipase A2 (5 times) were noted. Positive correlations were established between lipid peroxidation, SMP, and phospholipase (r = 0.63–0.78). Conclusion: ADH is accompanied by progressive OS, depletion of AOS, and an increase in EI.
References
Bjelakovic, G., Nikolova, D., & Gluud, C. (2021). Meta-analysis: antioxidant supplements for liver diseases – the Cochrane review. J Hepatol, 75(2), 474–482.
Chen, L., Zhao, Y., & Yang, Z. (2021). N-acetylcysteine attenuates oxidative stress and inflammatory response in biliary obstruction-induced liver injury. J Surg Res, 263, 200–208.
Chen, Y., Ji, L., Wang, Z., & Li, W. (2023). Bile Acids Induce Mitochondrial ROS, Activate NLRP3 Inflammasome and Contribute to Cholestatic Liver Injury via CamKII. J Hepatol, 78(2), 301–315.
Garcia, P., Martinez, A., & Flores, C. (2022). Pro-inflammatory cytokines (TNF-α, IL-1β, IL-6) as amplifiers of mitochondrial ROS generation in human gallbladder fibroblasts: implications for the pathogenesis of acute cholecystitis. Biochim Biophys Acta Mol Basis Dis, 1868(2), 166289.
Gromova, O.A., Tashkinov, V.A., Semenov, V.V., & Petrov, S.V. (2021). Molecular and cellular mechanisms of endogenous intoxication in purulent-inflammatory diseases of the abdominal cavity (review). Sovrem Tehnol Med, 13(2), 84–95.
Grotz, M.R.W., Dejong, C.H.C., & Lygidakis, N.J. (2021). The role of the gut in the development of sepsis in acute biliary disease. J Gastrointest Surg, 25(5), 1324–1331.
Ito, T., Yoshikawa, N., Inagaki, M., Miyazaki, T., & Machida, K. (2022). Secondary bile acids, gut microbiota, and experimental cholecystitis: Unraveling the pathogenic link. Hepatol Commun, 6(5), 1055–1069.
Kowalski, A., Szyluk, K., Kaczmarzyk, A., & Iłżecki, M. (2021). The Concentration of Middle Molecular Weight Substances in the Blood as a Prognostic Marker of the Severity of Acute Calculous Cholecystitis. Mediators Inflamm, 2021, 6621074.
Lee, J., Park, S., & Cho, H. (2022). A Novel Rat Model of Acute Obstructive Cholecystitis with Ischemia-Reperfusion Injury. In Vivo, 36(3), 1140–1147.
Li, F., Wang, D., Yu, Z., Li, Y., & Wang, J. (2022). Evaluation of oxidative stress and antioxidant capacity in patients with different severity of acute cholecystitis. Scand J Gastroenterol, 57(5), 602–609.
Li, S., Chen, J., Li, H., Zhang, Y., & Wang, Y. (2022). Oxidative Stress and Inflammatory Response in Acute Biliary Pancreatitis: A Comprehensive Review. Oxid Med Cell Longev, 2022, 5678251.
Liu, Y., Wang, Z., Li, J., & Zhang, L. (2021). The role of gut-derived endotoxemia and systemic inflammation in the progression of acute cholecystitis to sepsis. Hepatobiliary Pancreat Dis Int, 20(5), 449–455.
Okamoto, K., Suzuki, K., Takada, T., Strasberg, S.M., Asbun, H.J., Endo, I., et al. (2018). Tokyo Guidelines 2018: flowchart for the management of acute cholecystitis. J Hepatobiliary Pancreat Sci, 25(1), 55–72.
Petrov, S.V., Gromova, O.A., & Semenov, V.V. (2023). Experimental modeling of destructive cholecystitis: a review of existing methods and their pathogenetic validity. Bull Exp Biol Med, 175(1), 106–112.
Petrov, S.V., Semenov, V.V., & Gromova, O.A. (2023). Correction of oxidative stress and endotoxemia with mexidol in experimental peritonitis. Bull Exp Biol Med, 175(4), 468–472.
Sies, H., & Jones, D.P. (2020). Reactive oxygen species (ROS) as pleiotropic physiological signalling agents. Nat Rev Mol Cell Biol, 21(7), 363–383.
Smirnov, A.A., Ivanov, I.V., & Kozlov, I.A. (2023). Combined Use of Hemodialysis and High-Volume Hemofiltration with Adjuvant Antioxidant Therapy in Severe Abdominal Sepsis: A Pilot Study. Gen Reanimatol, 19(2), 15–24.
Smith, A.J., Jones, B.C., & Brown, D.E. (2022). Plasma 8-OHdG as a Potential Biomarker for Disease Severity and Systemic Oxidative Stress in Acute Calculous Cholecystitis. J Surg Res, 270, 308–315.
Wang, C., Li, Y., & Zhang, X. (2021). A Reliable Rabbit Model of Acute Gangrenous Cholecystitis Induced by Combination of Lithogenic Diet and Bacterial Infection. Lab Anim Res, 37, 25.
Wang, L., Chen, J., Wang, B., & Wu, D. (2023). Malondialdehyde Acts as a Trigger of Sepsis by Promoting Neutrophil Extracellular Traps Formation and Aggravating Oxidative Stress Injury. Sci Rep, 13(1), 5102.
Zaitseva, O.S., Kukhtina, E.E., & Derevyanchenko, M.V. (2022). Efficacy of Enterosorption in the Complex Therapy of Patients with Acute Cholecystitis and Concomitant Endogenous Intoxication Syndrome. Eksp Klin Gastroenterol, (1), 45–51.
Zhang, H., Li, Q., Zhang, R., & Wang, Y. (2023). Gut Microbiota Dysbiosis and Increased Intestinal Permeability in Patients with Obstructive Cholangitis: The 'Second Hit' in Liver Injury. J Clin Transl Hepatol, 11(1), 78–86.
Zhang, X., Wu, Z., Liu, G., Zhang, H., & Li, Y. (2022). Neutrophil Extracellular Traps Exacerbate Oxidative Stress and Biliary Epithelial Cell Injury in Experimental Cholangitis. Front Immunol, 13, 901234.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2026 Таалайбек Беков , Юсупжан Уметалиев
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
