MORPHOMETRIC AND HISTOCHEMICAL CHANGES IN THE WALL OF THE JEJUNUM OF BROILERS WHEN FEEDING ENTEROSORBENT WITH A STATIC DIET

Authors

  • Elena Prosekova Russian state agrarian university – MTAA
  • Nadezhda Cherepanova Russian state agrarian university – MTAA
  • Alexandra Seryakova Russian state agrarian university – MTAA
  • Tursumbai Kubatbekov Russian state agrarian university – MTAA
  • Evgeniya Baranovich Russian state agrarian university – MTAA
  • Abdugani Abdurasulov Osh state university https://orcid.org/0000-0003-3714-6102

DOI:

https://doi.org/10.52754/16948696_2024_3(8)_5

Keywords:

Poultry farming, broilers, feeding, intestines, mucous membrane, goblet cells, sorbents, enterosgel

Abstract

2 groups were formed from the daily broilers of the Competitor cross. The control group received the basic diet. The experimental group received enterosorbent Enterosgel (0.008%) during the first 3 days with the basic diet. Histological studies were performed at the age of 24, 4 and 49 days. Neutral goblet cells (GC) were detected using the PAS reaction, acidic GC – alcyan blue. The number of GC in the field of view was calculated and the density of the GC location on an area of 1000 microns was determined. The size of the villi and crypt layer was also measured. At 4 days of age, the value of the villi layer decreased by 14.2% (P <0.05) and crypts by 25.7%. In villi, the density of acidic GC increases by 71.8% (P≤0.001), and in crypts, the density of neutral GC increases by 83.8% (P≤0.001) and 80.0% (P≤0.001), respectively. At the end of the experiment, there was no difference in the size of the mucous membrane layers, the density of neutral GC in the experimental group decreased by 33.4% and 33.5% with high confidence, and the amount of acidic GC in the experimental groups increased in villi and crypts by 13.5% (P <0.05) and 22.3% (P<0.01).

References

Гистологическое строение органов пищеварения бройлеров при использовании комплекса биодобавок / Н.Г. Черепанова, В. П. Панов, А. Э. Семак [и др.] // Зоотехния. – 2020. – № 1. – С. 21-24. – DOI 10.25708/ZT.2019.94.92.009.

Efficiency and safety of siliceous enterosorbents in the therapy of Helicobacter pylori-associated diseases of the upper gastrointestin aI íract / E. Tkachenko, E. B. Avalueva, E. V. Skazyvaeva [et al.] // Minerva Gastroenterologica e Dietologica. – 2016. – Vol. 62, No. 3 S1. – P. 1-5.

Гусейнов М.М. Энтеросорбция при острых кишечных инфекциях молодняка крупного рогатого скота // Ветеринарная медицина. – 2012. – №3-4. – С. 70-71.

Investigation of the adsorption capacity of the enterosorbent Enterosgel for a range of bacterial toxins, bile acids and pharmaceutical drugs / C.A. Howell, S.V. Mikhalovsky, E.N. Markaryan [et al.] // Sci Rep 9, 5629 (2019). https://doi.org/10.1038/s41598-019-42176-z DOI: https://doi.org/10.1038/s41598-019-42176-z

Markelov D.A., Nitsak O.V., Gerashchenko I.I. Comparative Study of the Adsorption Activity of Medicinal Sorbents. Pharm Chem J 42, 405–408 (2008). https://doi.org/10.1007/s11094-008-0138-2 DOI: https://doi.org/10.1007/s11094-008-0138-2

Comparative characterization of polymethylsiloxane hydrogel and silylated fumed silica and silica gel / VM Gun'ko, VV Turov, VI Zarko, [et al.] //Journal of colloid and interface science. – 2007. – Т. 308. – №. 1. – С. 142-156. doi:10.1016/j.jcis.2006.12.053 DOI: https://doi.org/10.1016/j.jcis.2006.12.053

Развитие бокаловидных клеток двенадцатиперстной кишки бройлеров при скармливании энтеросорбента со стартовым рационом / Е. А. Просекова, Н. Г. Черепанова, Е. В. Панина [и др.] // Вестник Ошского государственного университета. Сельское хозяйство: агрономия, ветеринария и зоотехния. – 2023. – № 2. – С. 122-127. – DOI 10.52754/16948696_2023_2_16. DOI: https://doi.org/10.52754/16948696_2023_2_16

Thai P, Loukoianov A, Wachi S, Wu R. Regulation of airway mucin gene expression. Annu Rev Physiol. 2008;70:405-29. DOI: 10.1146/annurev.physiol.70.113006.100441 DOI: https://doi.org/10.1146/annurev.physiol.70.113006.100441

Полякова Е. П. Метод изучения полостного пищеварения / Е. П. Полякова, Д. А. Ксенофонтов, А. А. Иванов // Экспериментальная и клиническая гастроэнтерология. – 2016. – № 12(136). – С. 110-114.

Kim YS, Ho SB. Intestinal goblet cells and mucins in health and disease: recent insights and progress. CurrGastroenterol Rep. 2010 Oct;12(5):319-30. DOI: 10.1007/s11894-010-0131-2. DOI: https://doi.org/10.1007/s11894-010-0131-2

Development of goblet intestinal cells of broilers in case of introducing Bacillus subtilis spores into the diet / E. A. Prosekova, V. P. Panov, A. E. Semak [et al.] //. – 2022. – Vol. 12, No. 3. – P. 333-338. DOI 10.31407/ijees12.341. DOI: https://doi.org/10.31407/ijees12.341

Andrianifahanana M, Moniaux N, Batra SK. Regulation of mucin expression: mechanistic aspects and implications for cancer and inflammatory diseases. Biochim Biophys Acta. 2006 Apr;1765(2):189-222. DOI: 10.1016/j.bbcan.2006.01.002. DOI: https://doi.org/10.1016/j.bbcan.2006.01.002

Z. Uni, A. Smirnov, and D. Sklan T. Pre- and Posthatch Development of Goblet Cells in the Broiler Small Intestine: Effect of Delayed Access to Feed// Poultry Science, 2003.-Vol.82 – P.320–327 DOI.org/10.1093/ps/82.2.320 DOI: https://doi.org/10.1093/ps/82.2.320

Понкратова Т.Ю. Морфометрическая характеристика двенадцатиперстной кишки цыплят-бройлеров кросса Росс 308 по истечении 15-х и 21-х суток постнатального периода / Т.Ю. Понкратова, В.В. Семченко // Вестник Омского государственного аграрного университета. – 2017. – № 1(25). – С. 109-114.

Geyra A, Uni Z, Sklan D. The effect of fasting at different ages on growth and tissue dynamics in the small intestine of the young chick. Br J Nutr. 2001;86(1):53-61. DOI: 10.1079/BJN200136. DOI: https://doi.org/10.1079/BJN2001368

Ежова О.Ю., Беляцкая Ю.Н., Абдурасулов А.Х., Казакбаева О.В., Ласыгин П.В., использование мяса птицы при производстве мясопродуктов, В сборнике: Национальные приоритеты развития агропромышленного комплекса. Материалы национальной научно-практической конференции с международным участием. 2023. С. 341-344.

Published

2024-09-25

How to Cite

Prosekova, E., Cherepanova, N., Seryakova, A., Kubatbekov, T., Baranovich, E., & Abdurasulov, A. (2024). MORPHOMETRIC AND HISTOCHEMICAL CHANGES IN THE WALL OF THE JEJUNUM OF BROILERS WHEN FEEDING ENTEROSORBENT WITH A STATIC DIET. Journal of Osh State University. Agriculture: Agronomy, Veterinary and Zootechnics, (3(8), 38–46. https://doi.org/10.52754/16948696_2024_3(8)_5

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