CRISPR/CAS9 ТУРУКТУУЛУГУН ЖАНА НАТЫЙЖАЛУУЛУГУН ЖОГОРУЛАТУУ ҮЧҮН NANODEVICE НЕГИЗДЕЛГЕН ГЕНОМ ТҮЗӨТҮҮ
DOI:
https://doi.org/10.52754/16948688_2022_1_8Ачкыч сөздөр:
crispr/cas, нанотехнология, crispr/cas9, жеткирүүАннотация
Он жыл мурун, ГЕНОМДУ өзгөртүү жаатында CRISPR жана CRISPR менен байланышкан тутумдун (Cas) мааниси жогорулаган. Бул ыкманын клетка ичиндеги жеткирүү эффективдүүлүгү анын ыңгайлуулугуна карабастан, кассалык жүктөрдү жана sgRNA ташууну кыйындатат. Наноматериалдар, анын ичинде липосомалар, полимерлер, алтын нанобөлүкчөлөр жана органикалык эмес нанобөлүкчөлөр генди өткөрүп берүү үчүн ийгиликтүү колдонулган. Бул жерде, биз кыскача көп CRISPR/Cas жеткирүү системаларын жана алардын байланышкан кыйынчылыктарды камтыйт, анда биз CRISPR/Cas жеткирүү үчүн ар кандай нанотехнологиялык жолдор менен барып, CRISPR негизделген өсүмдүк геном түзөтүү көптөгөн маселелерди жаратат. Ошондой эле, CRISPR/Cas9 Нанотехнологияны жана ушул түзөтүү ыкмасынан пайда алуу үчүн максаттуу болушу керек болгон аймактарды колдонуу кыйынчылыктарын камтыган.
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